Journal: Vaccines

Article Title: Placental Transfer Immunity to the Newborns in a Twin Pregnant Women Vaccinated with Heterologous CoronaVac-ChAdOx1

doi: 10.3390/vaccines11010116

Figure Lengend Snippet: Comparisons of immunization profiles between the mother and both twins.

Article Snippet: Additionally, serum-neutralizing antibodies against wild-type and delta (B.1.617.2) variants were determined using a surrogate virus neutralization test (cPassTM, GenScript, Piscataway, NJ, USA) according to the manufacturer’s instruction with modifications.

Techniques: Neutralization, Activity Assay, Inhibition

Journal: Journal of Virological Methods

Article Title: Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2

doi: 10.1016/j.jviromet.2021.114228

Figure Lengend Snippet: Sensitivity of the different serological tests and cut-offs used based on the final serum panels. Values between brackets represent 95 % confidence intervals on the estimations.

Article Snippet: Here, we performed an independent evaluation of a commercially available surrogate virus neutralization test (sVNT, GenScript cPassTM) that can be done without biosafety level 3 containment in less than 2 h. When using the cVNT and a Luminex multiplex immunoassay (MIA) as reference, the sVNT obtained a sensitivity of 94 % (CI 90–96 %) on a panel of 317 immune sera that were obtained from hospitalized and mild COVID-19 cases from Belgium and a sensitivity of 88 % (CI 81–93 %) on a panel of 184 healthcare workers from the Democratic Republic of Congo.

Techniques: Luminex

Journal: Journal of Virological Methods

Article Title: Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2

doi: 10.1016/j.jviromet.2021.114228

Figure Lengend Snippet: Correlations between the percentage of inhibition measured by the surrogate viral neutralisation test (sVNT) and the log(dilution factors or signal-to-noise ratio) for the conventional viral neutralization test (cVNT) or the Luminex multiplex immunological assay (MIA) as calculated by the nonparametric Spearman correlation test (r s ). Seropositivity cut-off levels for the sVNT are indicated by the dashed grey lines at 20 or 30 % inhibition. Negative samples on the cVNT or MIA were not included in these figures.

Article Snippet: Here, we performed an independent evaluation of a commercially available surrogate virus neutralization test (sVNT, GenScript cPassTM) that can be done without biosafety level 3 containment in less than 2 h. When using the cVNT and a Luminex multiplex immunoassay (MIA) as reference, the sVNT obtained a sensitivity of 94 % (CI 90–96 %) on a panel of 317 immune sera that were obtained from hospitalized and mild COVID-19 cases from Belgium and a sensitivity of 88 % (CI 81–93 %) on a panel of 184 healthcare workers from the Democratic Republic of Congo.

Techniques: Inhibition, Neutralization, Luminex, Multiplex Assay

Journal: Frontiers in Immunology

Article Title: Design and performance characteristics of the Elecsys anti-SARS-CoV-2 S assay

doi: 10.3389/fimmu.2022.1002576

Figure Lengend Snippet: Correlation of the ACOV2S assay with results from the cPass neutralization assay. (A) ACOV2S titers and percentage inhibition from the cPass assay from longitudinal samples from exemplary selected individual donors, and (B) comparison of the ACOV2S and cPass results stratified in time buckets after diagnostic PCR and (C) derived qualitative agreements. ACOV2S reactivity in samples with cPass inhibitory capacities is shown as positive percent agreement (PPA), cPass inhibitory capacity ACOV2S reactive samples is shown as positive predictive value (PPV). Analyses were carried out applying both, 0.8 U/mL and 15 U/mL as decision point for relevant ACOV2S reactivity. This analysis used native samples collected from convalescent donors at different timepoints after infection.

Article Snippet: The correlation of ACOV2S with a surrogate neutralization assay (cPassTM, Genscript) has already been described ( ).

Techniques: Neutralization, Inhibition, Comparison, Diagnostic Assay, Derivative Assay, Infection